教員紹介

小俣 義明
Omata Yoshiaki

所属・肩書

健康薬学科/分子生物学研究室(教授)

最終学歴

東京理科大学大学院 薬学研究科 博士課程

取得学位

博士(薬学) (東京理科大学)

所属学会

日本生化学会、日本分子生物学会、日本薬学会、日本蛋白質科学会、日本生物物理学会

現在の専門分野

酵素反応機構、生物物理学、分子生物学、ヘムタン白質

現在の研究課題

●ヘムの代謝に関わる酵素の反応機構
●タン白質の結晶化と構造活性相関
●味と匂いの分子認識機構

主要研究業績

●tRNAに存在する修飾塩基の役割
●薬物代謝酵素の精製及び機能解析
●サイトクロームP450の物理化学的性質及び関連タン白質との相互作用
●ヘム代謝関連酵素の構造と活性の関係
●天然ポルフィリンの側鎖が非対称であることの生理的意義

最近の研究業績

1.Transcriptional factor Fur from Thermoplasma volcanium binds its own promoter DNA in a divalent cation-dependent manner, Yu Ikeda, Hikari Minoshima, Mai Satoh, Tomoaki Ishikawa, Yoshie Kawashima-Ohya, Koji Tomobe, Yoshiaki Omata, and Tsuyoshi Kawashima, J. Gen. Appl. Microbiol., 58, 465-473 (2012)

2.Purification and Characterization of Human Uroporphyrinogen III Synthase Expressed in Eschericia coli., Yoshiaki Omata, Hiroshi Sakamoto, Yuichiro Higashimoto, Shunsuke Hayashi, and Masato Noguchi, J. Biochem., 13(2), 211-220 (2004)

3.Characterization of Rat Heme Oxygenase-3 Gene. Implication of Processed Pseudogenes Derived from Heme Oxygenase-2 Gene, Shunsuke Hayashi, Yoshiaki Omata, Hiroshi Sakamoto, Yuihiro Higashimoto, Takayuki Hara, Yasuhiro Sagara, and Masato Noguchi, Gene, 336(2), 241-250 (2004)

4.Purification and Characterization of a Soluble Form of Rat Liver NADPH-Cytochrome P450 Reductase Highly Expressed in Escherichia coli., Shunsuke Hayashi, Yoshiaki Omata, Hiroshi Sakamoto, Takayuki Hara, and Masato Noguchi, Protein Expr. Purif., 29(1), 1-7 (2003)

5.Crystal Structure of Rat Heme Oxygenase-1 in Complex with Heme Bound to Azide: Implication for Regiospecific Hydroxylation of Heme at the a-meso Carbon, Masakazu Sugishima, Hiroshi Sakamoto, Yuichiro Higashimoto, Yoshiaki Omata, Shunsuke Hayashi, Masato Noguchi, and Keiichi Fukuyama, J. Biol. Chem., 277(47), 45086-45090 (2002)

6.The Reactivity of a-Hydroxyhaem and Verdohaem Bound to Haem Oxygenase-1 to Dioxygen and Sodium Dithionite, Hiroshi Sakamoto, Yoshiaki Omata, Shunsuke Hayashi, Saori Harada, Graham Palmer, and Masato Noguchi, Eur. J. Biochem., 269(21), 5231-5239 (2002)

7.Crystal Structure of Rat Apo-heme Oxygenase-1 (HO-1): Mechanism of Heme Binding in HO-1 Inferred from Structual Comparison of the Apo and Heme Complex Forms, Masakazu Sugishima, Hiroshi Sakamoto, Yoshimitsu Kakuta, Yoshiaki Omata, Shunsuke Hayashi, Masato Noguchi, and Keiichi Fukuyama, Biochemistry, 41(23), 7293-7300 (2002)

8.Separation and Identification of the Regioisomers of Verdoheme by Reversed-phase Ion-pair High-performance Liquid Chromatography, and Charcterization of Their Comlexes with Heme Oxygenase, Hiroshi Sakamoto, Yoshiaki Omata, Yoshio Adachi, Graham Palmer, and Masato Noguchi, J. Inorg. Biochem., 82(1-4), 113-121 (2000)

9.Crystal Structure of a Rat Heme Oxygenase-1 in Complex with Heme, Masakazu Sugishima, Yoshiaki Omata, Yoshimitsu Kakuta, Hiroshi Sakamoto, Masato Noguchi, and Keiichi Fukuyama, FEBS Lett., 471(1), 61-66 (2000)

10.Synthetic Peptide Mimics of a Predicted Topographical Interaction Surface: The Cytochrome P450 2B1 Recognition Domain for NADPH-Cytochrome P450 Reductase, Yoshiaki Omata, Renke Dai, Stanley V. Smith, Richard C. Robinson, and Fred K. Friedman, J. Protein Chem., 19(1), 23-32 (2000)

11.Ferric a-Hydroxyheme Bound to Heme Oxygenase Can Be Converted to Verdoheme by Dioxygen in the Absence of Added Reducing Equivalents, Hiroshi Sakamoto, Yoshiaki Omata, Graham Palmer, and Masato Noguchi, J. Biol. Chem., 274(26), 18196-18200 (1999)

12.Crystallization and Preliminary X-ray Diffraction Studies on the Water Soluble Form of Rat Heme Oxygenase-1 in Complex with Heme, Yoshiaki Omata, Shinya Asada, Hiroshi Sakamoto, Keiichi Fukuyama, and Masato Noguchi, Acta Cryst., D54, 1017-1019 (1998)

13.cDNA-Directed Dxpression of Human Cytochrome P450 3A4 Using Baculovirus, Jeroen T. M. Buters, Kenneth R. Korzekwa, Kent L. Kunze, Yoshiaki Omata, James P. Hardwick, and Frank J. Gonzalez, Drug Metabol. Dispos., 22(5), 688-692 (1994)

14.Interaction Between Cytochrome P450 2B1 and Cytochrome b5: Inhibition by Synthetic Peptides Indicates a Role for P450 Residues Lys-122 and Arg-125, Yoshiaki Omata, Hiroshi Sakamoto, Richard C. Robinson, Matthew R. Pincus, and Fred K. Friedman, Biochem. Biophys. Res. Commun., 201(3), 1090-1095 (1994)

15.Specificity of the Cytochrome P450 Interaction with Cytochrome b5, Yoshiaki Omata, Richard C. Robinson, Harry V. Gelboin, Matthew R. Pincus, and Fred K. Friedman, FEBS Lett., 346(2,3), 241-245 (1994)

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